Cuban Journal of Forest Sciences. 2022; January-April 10(1): 31-43
Translated from the original in spanish
Morpho-physiological response of Ochroma pyramidale produced in nurseries by biopot technology to N, P, K fertilisation using an optimal custom design
Respuesta morfofisiológica de Ochroma pyramidale producida en viveros mediante tecnología biopot a la fertilización con N, P, K utilizando un diseño óptimo personalizado
Resposta morfofisiológica da pirâmide Ochroma pyramidale cultivada em viveiros empregando a tecnologia de fertilização N, P, K com um projeto ideal feito sob medida
Yasiel Arteaga-Crespo1 https://orcid.org/0000-0002-9817-9883
Yudel García-Quintana1 https://orcid.org/0000-0002-9107-9310
Carlos Alfredo Bravo-Medina1 https://orcid.org/0000-0002-8746-7900
Diego Armando Ureta-Leones2 https://orcid.org/0000-0003-1036-7642
1Amazonian State University. Ecuador.
2Ministry of Environment, Water and Ecological Transcition (MAATE) Quito, Ecuador.
*Corresponding author: firstname.lastname@example.org
Ochroma pyramidale is a fast-growing species with high commercial value. The largest volumes of its wood are obtained from commercial plantations; however, there are very few studies of its production in nurseries. The objective of this study was analyse the effect of N, P, K concentration and fertilisation frequency on morpho-physiological response of O. pyramidale grown in biopots using response surface methodology. The experiment was set up using the response surface methodology for which 30 seedlings were used per experimental run. The experiment consisted of applying different concentrations of N, P, K and two fertilisation frequencies according to the optimal (Custom) design. The response variables were height increase, diameter increase and CO2 assimilation. From the results obtained, we confirmed the positive effect of N, P and K fertilisation on the variables under study. From the experimental data, a second-order quadratic polynomial model was found that allows the prediction of the morpho-physiological response of the species. The frequency of fertilisation during the time interval studied was not significant.
Keywords: fertilisation; response surface methodology; Ochroma pyramidale.
Ochroma pyramidale es una especie de rápido crecimiento y alto valor comercial. Los mayores volúmenes de su madera se obtienen de plantaciones comerciales; sin embargo, existen muy pocos estudios sobre su producción en vivero. El objetivo de este estudio fue analizar el efecto de la concentración de N, P, K y la frecuencia de fertilización sobre la respuesta morfofisiológica de O. pyramidale cultivada en tubetes utilizando la metodología de superficie de respuesta. El experimento se estableció utilizando la metodología de superficie de respuesta para lo cual se utilizaron 30 plántulas por corrida experimental. El experimento consistió en aplicar diferentes concentraciones de N, P, K y dos frecuencias de fertilización según el diseño óptimo (personalizado). Las variables de respuesta fueron el aumento de altura, el aumento de diámetro y la asimilación de CO2. A partir de los resultados obtenidos, se confirmó el efecto positivo de la fertilización con N, P y K sobre las variables en estudio. A partir de los datos experimentales se encontró un modelo polinómico cuadrático de segundo orden que permite predecir la respuesta morfofisiológica de la especie. La frecuencia de fertilización durante el intervalo de tiempo estudiado no fue significativa.
Palabras clave: Fertilización; Metodología de superficie de respuesta; Ochroma pyramidale.
A Ochroma pyramidale é uma espécie de crescimento rápido com alto valor comercial. Os maiores volumes de sua madeira são obtidos de plantações comerciais; no entanto, há muito poucos estudos sobre sua produção em berçário. O objetivo deste estudo foi analisar o efeito da concentração de N, P, K e frequência de fertilização na resposta morfofisiológica da pirâmide de O. pyramidale cultivada em biopots usando a metodologia de superfície de resposta. O experimento foi implementado utilizando a metodologia da superfície de resposta, para a qual foram utilizadas 30 mudas por série experimental. A experiência consistiu na aplicação de diferentes concentrações de N, P, K e duas frequências de fertilização de acordo com o projeto ideal (Personalizado). As variáveis de resposta foram aumento da altura, aumento do diâmetro e assimilação de CO2. A partir dos resultados obtidos, o efeito positivo da fertilização N, P e K sobre as variáveis em estudo foi confirmado. A partir dos dados experimentais, foi encontrado um modelo polinomial quadrático de segunda ordem para prever a resposta morfofisiológica da espécie. A frequência da fertilização durante o intervalo de tempo estudado não foi significativa.
Palavras-chave: Fertilização; Metodologia de superfície de resposta; Ochroma pyramidale.
Ochroma pyramidale (balsa) is a species native to the Americas, ranging from southern Mexico to Peru. It is a fast-growing species that reaches 20m in height and up to 75cm in diameter in 5-8 years. Given the physical and mechanical properties of its wood, it has been widely used for different purposes such as toys, handicrafts, interior veneer, insulation and pulp for paper (Borrega et al., 2015). Therefore, balsa wood is in high demand in the international market, mainly in Europe, China and the United States. It is also used in restoration projects for degraded areas (Miyajima et al. 2018; Cañadas-López et al., 2019).
In its natural habitat, the species occurs as individual trees in forest clearings in tropical rainforests and in mixed groups with other species. It rarely grows in dense stands (Fletcher 1951). The high dispersal of balsa over large areas of forest makes extraction difficult and costly. For this reason, most of the commercially used timber is from plantations, particularly from Ecuador (Borrega et al., 2015).
Despite the demand for the species, there has not been a great deal of much research into the management and production of the species, so further studies are needed. In this regard, fertilisation in nurseries to obtain nourished seedlings that respond better to field conditions is a topic that remains a novelty. Many authors argue that fertilisation in containers at the nursery stage increases survival by improving the plants' physiological state during the establishment phase through increased root growth (Luis et al., 2009; Trubat et al., 2010). Therefore, the objective of this research was to analyse Ochroma pyramidale produced in nurseries using biopot technology with the aim of analysing the effect of N, P, K concentration and fertilisation frequency on morpho-physiological response of O. pyramidale grown in biopots using response surface methodology (RSM).
RSM has been used in several areas of research due to the advantages it offers, as it considerably reduces the experimental runs. In addition, it allows one to find a predictive model of the independent variables from the study factors.
MATERIALS AND METHODS
Location of the experiment
The experiment was carried out in the nursery located in the central campus of the Universidad Estatal Amazónica, Puyo, Ecuador (1° 27' 59.8'' S; 77° 59' 51.6'' W) at an average maximum and minimum temperature inside the nursery of 28 °C and 17 °C (Davis Vantage Pro 2 weather station), respectively.
Biological material and experimental conditions
Seeds were collected from O. pyramidale trees in Arajuno parish, Pastaza, Ecuador (1° 13' 01.8'' S; 77° 39' 08.5'' W, 1° 15' 58.1'' S; 77° 37' 31.4'' W, 1° 16' 27.4'' S; 77° 41' 20.3'' W). Subsequently, in the laboratory, the seeds were selected according to their morphology and placed in a container with water to eliminate the empty seeds. They were disinfected with 0.5% sodium hypochlorite and soaked for 24 hours as a pre-germinative treatment. Next, they were sown directly in earthworm humus with a composition of 2 % nitrogen, 0.48% phosphorus and 1.13 % potassium, and a pH of 7.08. In addition, 10 % (v/v) of rice husk was added to encourage aeration. Seedling production was carried out in black biopots of 110 cm-3. Fertilisation was initiated when the seedlings reached three months of age (30 seedlings per experimental run). Three fertilizer were used as independent source of N, P and K. Nitrogen source was urea (46 % N), rock phosphate (28 % P) and potassium chloride (60 % K) respectively.
At fourth month from the cultivation was assessed the response to increase height (cm), diameter (mm) and CO2 assimilation (ìmol m-2s-1). In case of photosynthetic assimilation rate (A) was measured with a portable integrated photosynthesis and chlorophyll fluorescence measurement system with fully programmable microclimate control (iFL/Cpro-SD, ADC BioScientific Ltd., Herts, UK). Determinations were performed between 8:00 h and 11:00 h on ten seedlings, as proposed by Avila-Lovera & Tezara (2018) and Avila-Lovera et al., (2019), on mature, intact leaves. The operating conditions were ambient CO2 (~ 400ìmol mol-1) assisted by a compressed CO2 cylinder, 21 % O2, Photosynthetic photon flux density 1000ìmol m-1s-1 leaf temperature similar to ambient 25.4 ± 0.2°C and a vapour pressure deficit of 4.36 ± 0.4 kPa.
Optimal (custom) design
Using Design Expert software version 12.0 (serial number 9847-9696-7992-6750, Stat-Ease Inc., 1300 Godward Street North, Suite 6400 Minneapolis, USA), the optimal (custom) design (Table 1) was performed with N, P, K concentration as a numerical factor and fertilisation frequency as a categorical factor. The range of N, P, K concentrations (75-150 mg L-1) in the experiment was selected according to the trials conducted by Basave-Villalobos et al., (2020). ANOVA was applied to analyse the influence of N, P, K concentration on height increase, diameter increase and CO2 assimilation of O. pyramidale as independent variables (P <0.05). The veracity of the model was determined by the coefficient of determination (R2) and significance (P) (Table 1).
Table 1. - Optimal (custom) design for the independent variables (N, P, K concentration
and fertilisation frequency) and experimental and predicted results of height increase,
diameter increase and CO2 assimilation (CO2 A.) of Ochroma pyramidale
In order to analyse the effect of N, P, K concentration and fertilisation frequency on height increase, diameter increase and CO2 assimilation of O. pyramidale grown in biopots, fourteen experimental runs were carried out, starting from the optimal (custom) design, which allows the highest increase in the variables considered. Table 1 shows the experimental results and those predicted for the construction of the model. Increases in height were between 1.32 and 4.53cm and in diameter were between 0.8 and 1.29mm respectively. Regarding assimilation rates, the species presented minimum values of 5.3ìmol m-2s-1 and maximum values of 15.2ìmol m-2s-1. Of the models predetermined by the design (Table 2), it was found that = the best fit for all variables was the quadratic model with an R2= 0.9257 (increase in height), R2= 0.9413 (increase in diameter) and 0.9361 (CO2 assimilation). These results indicate that 92.5 %, 94.1 % and 93.6 % of the total variation of the variables was determined by the concentration of N, P, K applied (Table 2).
Table 2. - Summary of the polynomial models analysed by the Design Expert Software on
the effect of N, P, K concentration and fertilisation frequency on height increase,
diameter increase and CO2 assimilation of Ochroma pyramidale grown in biopots
Figure 1 (A, C and E) shows the predicted and experimental values 120 days after germination for height increase, diameter increase and CO2 assimilation of O. pyramidale grown in biopots. The distribution of points corroborated the ability of the model to cover the whole experimental range. The R2 and adjusted R2 values of the regression lines are close to one, indicating a very good correspondence between the experimental and predicted values of the model on the experimental data. Figure 1 (B, D and F) shows that the experimental data met the assumption of normal distribution (Figure 1).
Figure 1. - Experimental versus predicted values for optimal (custom) design: (A) increase
in height; (C) increase in diameter;
(E) CO2 assimilation; (B, D and F) Normal distribution of experimental data
Also, according to the results in Table 3, it was confirmed that the concentration of N, P, K was a significant factor (P <0.05) in the morpho-physiological response of the species for the three response variables, whilst the frequency of fertilisation for the period evaluated was not significant (P >0.05).
Table 3. - ANOVA for Response Surface Quadratic model
Based on the height increase, diameter increase, CO2 assimilation of O. pyramidale and the desirability function, a second-order quadratic polynomial regression equation was established in terms of coded values (Eq. 1, Eq. 2 and Eq. 3 below). According to this model, the dependency relationship for each study variable and the applied N, P, K concentrations can be predicted. The graphical representations of the regression equations are shown in Figure 2 (B, C and D). The statistical significance of the regression equations concerning the polynomial model was tested using the F-test and ANOVA (Table 3) and (Figure 2). (Equation 1); (Equation 2) and (Equation 3).
Figure 2. - Model graph for optimal (custom) design: (A) desirability; (B) height increase;
(C) diameter increase; (D) CO2 assimilation;
(E) correlation between N, P, K concentration and response variables
Cultural fertilisation practices in the nursery are important in the cultivation of container-grown seedlings. These practices have a strong influence on the morphological development and nutrient levels of the seedlings (Villar-Salvador et al., 2009). The results obtained in our investigation show that with higher concentrations of N, P and K, biomass production was favoured. This was reflected in the increases in height and diameter as a consequence of greater CO2 assimilation, given the high correlation found between the variables (Figure 3E).
The positive effect of N, P, K fertilisation has been documented by various authors. For example, Hawkins et al., (2005) reported that applying nutrients to Tsuga heterophylla (Raf.) Sarg. seedlings improved the growth. Other authors stated that nitrogen, phosphorus and potassium are the three primary macronutrients that favour photosynthetic rate, root growth and efficient water use, respectively (Fernández et al., 2006). Oliet et al., (2005) argued that cultural regimes at the nursery stage influence plant quality. They examined the influence of mineral nutrition at the nursery stage on Acacia salicina Lindl. that was planted on a degraded site in southeastern Spain. They found that survival was significantly higher for seedlings fertilised at high rates and that the initial benefits to field growth associated with nursery fertilisation diminished after four years. The survival of nourished seedlings was significantly higher than that of non-nourished seedlings after prolonged drought after the sixth year. The results suggest that the mineral nutrient status of nursery material (especially high P content) may positively affect the long-term establishment of A. salicina seedlings in semi-arid Mediterranean climates.
It has also been found that the fertilisation of Quercus ilex seedlings in nurseries influences morphophysiology and yield in the field (Andivia et al., 2011). The fertilisation of Pinus tabuliformis Carr. in nurseries also indicated positive effects on seedling growth (Shi et al., 2019). In an investigation of alternative substrates and fertiliser doses in the production of Pinus cembroides Zucc. in nurseries, the authors concluded that the use of a higher fertiliser dose, regardless of the substrates used, promoted higher plant growth responses (Madrid-Aispuro et al., 2020). In another experiment to analyse the influence of mineral fertilisation in different growing media on the growth of Inga edulis Mart seedlings, the positive effect of fertilisation was also demonstrated (Mahmoud and Hussein 2021).
In a study on nutrient content and photosynthesis in young O. pyramidale plants carried out in the central Brazilian Amazon, it was found that with adequate foliar levels of nitrogen and phosphorus, the CO2 assimilation rate was 13ìmol m-2s-1 (Marenco et al., 2001). The values found in our investigation for the highest dose of N, P, K used agree with those reported by these authors and corroborate the physiological response of the species to fertilisation.
Using the response surface methodology, a second-order quadratic polynomial relationship was found between the independent variables (concentration of N, P, K and frequency of fertilisation) and the dependent variables (height increase, diameter increase and carbon dioxide assimilation). In the interval studied, the frequency of fertilisation was not significant, so the use of a single initial dose is better. The higher concentration of nutrients applied had an effect on the increase in height and diameter, as well as the higher rate of CO2 assimilation.
ANDIVIA, E., FERNÁNDEZ, M., AND VÁZQUEZ-PIQUÉ, J. 2011. Autumn fertilization of Quercus ilex ssp. ballota (Desf.) Samp. nursery seedlings: Effects on morpho-physiology and field performance. Ann. For. Sci. [en línea] vol. 68 no 3: 543-553. doi:10.1007/S13595-011-0048-4.
BORREGA, M., AHVENAINEN, P., SERIMAA, R., y GIBSON, L. 2015. Composition and structure of balsa (Ochroma pyramidale) wood. Wood Sci. Technol. [en línea] vol. 49 no.2: pp. 403-420. doi:10.1007/s00226-015-0700-5.
CAÑADAS-LÓPEZ, Á., RADE-LOOR, D., SIEGMUND-SCHULTZE, M., MOREIRA-MUÑOZ, G., VARGAS-HERNÁNDEZ, J.J., y WEHENKEL, C. 2019. Growth and yield models for balsa wood plantations in the coastal lowlands of Ecuador. Forests [en línea] vol. 10 no. 9. doi:10.3390/f10090733.
FERNÁNDEZ, M., NOVILLO, C., AND PARDOS, J.A. 2006. Effects of water and nutrient availability in Pinus pinaster Ait. open pollinated families at an early age: Growth, gas exchange and water relations. New For. [en línea] vol. 31 no. 3: pp. 321-342. doi:10.1007/s11056-005-8196-8.
FLETCHER, M.I. 1951. Balsa Production and utilization. Econ. Bot. [en línea] vol. 5, no. 2. pp. 107-125. doi:10.1007/BF02984770.
HAWKINS, B.J., BURGESS, D., y MITCHELL, A.K. 2005. Growth and nutrient dynamics of western hemlock with conventional or exponential greenhouse fertilization and planting in different fertility conditions. Can. J. For. Res. [en línea] vol. 35 no. 4: pp.1002-1016. doi:10.1139/x05-026.
LUIS, V.C., PUÉRTOLAS, J., CLIMENT, J., PETERS, J., GONZÁLEZ-RODRÍGUEZ, Á.M., MORALES, D., ANDy JIMÉNEZ, M.S. 2009. Nursery fertilization enhances survival and physiological status in Canary Island pine (Pinus canariensis) seedlings planted in a semiarid environment. Eur. J. For. Res. [en línea] vol. 28 no. 3: pp. 221-229. doi:10.1007/s10342-009-0257-7.
MADRID-AISPURO, R.E., PRIETO-RUÍZ, J.Á., ALDRETE, A., HERNÁNDEZ-DÍAZ, J.C., WEHENKEL, C., CHÁVEZ-SIMENTAL, J.A., AND MEXAL, J.G. 2020. Alternative substrates and fertilization doses in the production of Pinus cembroides Zucc. in nursery. Forests [en línea] vol. 11 no. 1. doi:10.3390/f11010071.
MAHMOUD, A.M.M., AND HUSSEIN, N.R. 2021. Influence of mineral fertilization and different growing media on the growth and chemical composition of Inga edulis Mart. tree seedling. svuijas.journals.ekb.eg [en línea] vol. 3 no. 3: pp. 16-29. doi:10.21608/SVUIJAS.2021.68858.1094.
MARENCO, R.A., DE, J.F., y VIEIRA, G. 2001. Photosynthesis and leaf nutrient contents in Ochroma pyramidale (Bombacaceae). Photosynthetica [en línea] vol. 39 no. 4: pp. 539-543. doi:10.1023/A:1015699927924.
MIYAJIMA, R., VITÓRIA CASTRO SANTOS BARRETO, PAULO ANDRÉ DE OLIVEIRA, GISLAINE CRISTINA BATISTELA, AND DANILO SIMÕES. 2018. Risk Analysis of the Economic Benefits of Ochroma pyramidale: A Case Study of Forest Planting in Brazil. J. Agric. Sci. Technol. B [en línea] vol. 8, no. 7. doi:10.17265/2161-6264/2018.07.004.
OLIET, J.A., PLANELLES, R., ARTERO, F., AND JACOBS, D.F. 2005. Nursery fertilization and tree shelters affect long-term field response of Acacia salicina Lindl. planted in Mediterranean semiarid conditions. For. Ecol. Manage. [en línea] vol. 215 no. 13. pp. 339-351. doi:10.1016/j.foreco.2005.05.024.
SHI, W., GROSSNICKLE, S.C., LI, G., SU, S., y LIU, Y. 2019. Fertilization and irrigation regimes influence on seedling attributes and field performance of Pinus tabuliformis Carr. Forestry [en línea] vol. 92 no. 1: pp. 97-107. doi:10.1093/forestry/cpy035.
TRUBAT, R., CORTINA, J., AND VILAGROSA, A. 2010. Nursery fertilization affects seedling traits but not field performance in Quercus suber L. J. Arid Environ. [en línea] vol. 74 no. 4: pp. 491-497. doi:10.1016/j.jaridenv.2009.10.007.
VILLAR-SALVADOR, P., PUÉRTOLAS, J., y PEÑUELAS, J.L. 2009. Assessing Morphological and Physiological Plant Quality for Mediterranean Woodland Restoration Projects. L. Restor. to Combat Desertif. Innov. Approaches, Qual. Control Proj. Eval. [en línea] (Burdett 1990): pp. 103-120. Available from: http://www.research.lancs.ac.uk/portal/en/publications/assessing-morphological-and-physiological-plant-quality-for-mediterranean-woodland-restoration-projects(3769e750-2995-43ac-a859-dc65fc4b0041).html
Conflict of interests:
The authors declare not to have any interest conflicts.
The authors have participated in the writing of the work and analysis of the documents.
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Copyright (c) 2022 Yasiel Arteaga-Crespo, Yudel García-Quintana, Carlos Alfredo Bravo-Medina, Diego Armando Ureta-Leones