The importance of soil seed banks in ecological studies

Revista Cubana de Ciencias Forestales. September-December, 2019, 7(3): 276-282

 

Translated from the original in spanish

 

The importance of soil seed banks in ecological studies

 

La importancia de los bancos de semillas del suelo en los estudios ecológicos

 

Pablo Ferrandis

Jardín Botánico de Castilla-La Mancha y E.T.S. Ingenieros Agrónomos y de Montes, Campus Universitario s/n, 02071 Albacete, España.
E-mail: pablo.ferrandis@uclm.es


A population and community perspective

The soil seed bank is the term ecologists use to designate the set of viable seeds contained in soil, on the surface, buried or associated with mulch (Leck et al. 1989).  Seed banks play a fundamental role in the dynamics of plant populations and communities.  From a functional point of view, one of the most relevant aspects is the time that seeds can remain in the soil, as this will determine the amount of reserves that species will have at any given time to attend to recruitment under regular environmental conditions or unforeseen situations of disturbance on a small or large spatial scale.

This aspect becomes particularly critical in the case of rare or threatened taxa (Ferrandis et al., 2011a, 2011b). The classic work of Thompson and Grime (1979) represented a milestone in the ecology of seed banks, proposing for the first time a classification in this sense: (i) transitory banks, those of species whose soil seed reserves are depleted before a new phenological cycle is completed, i.e., before a new episode of seed dispersal occurs, and (ii) persistent banks, those in which a significant fraction remains viable in the soil for more than one phenological cycle, overlapping the dispersal of the new crop. Subsequently, Thompson et al. (1997) refined the classification from the proposals of Bakker (1989) and Bakker et al. (1991), identifying three large groups from the functional point of view: (i) transitory, for species whose seed banks persist in the soil for less than one year (coinciding in general with the duration of a phenological cycle); (ii) persistent of short duration, for species whose seeds persist in the soil for more than one year, but less than five years; (iii) persistent of long duration, to define those seed banks capable of persisting in the soil for more than five years.

Obviously, the five-year border is still conventional, but it fits in well with the reality of the abundant database that the authors created on seed banks in Europe, based on the research published up to that date. Each type of seed bank has been modelled by natural selection and acts as an efficient strategy under the conditions in which the species live. In fact, one might think that under a regime of predictable disturbances in time and space (e.g., summer exhaustion of herbaceous plants in Mediterranean environments), a transient seed bank, formed shortly before they occur, results in the most efficient investment-benefit strategy (Thompson and Grime 1979, Ferrandis et al. 2001). In contrast, in the face of a regime of unpredictable disturbances, the evolutionary solution will be directed towards the accumulation of seeds in the soil (Thompson and Grime, 1979).

In ecosystems subject to recurrent fires, such as many Mediterranean shrubs, large persistent seed banks play a significant role in plant regeneration (Ferrandis et al., 1999a). Similarly, this is why persistent seed banks predominate in the early stages of ecological succession, while the pseudo-stable and mature stages of vegetation are dominated by species with transient banks (Baskin and Baskin 1998, Fenner, 2000). In today's changing world, with the modification that human activity is driving on a large scale through global change, persistent seed banks can play a relevant role in the resilience of populations and communities: according to the classification of Thompson et al. (1997) and its functional interpretation, short-term persistent banks would decisively drive the maintenance of populations, while long-term persistent banks would have definitive consequences on the resilience of communities. The study of soil seed banks, either in focused work on the populations of a given species or in entire plant communities, can provide crucial information for the accurate interpretation of the state, responses and dynamics of them.

Basic guide for soil seed bank analysis protocols. Sampling

The collection of soil samples (blocks) in the environment where it is wanted to evaluate the seed bank can be done with the help of a cylindrical metallic probe (3-5 cm in diameter) or a gardening shovel.

The tool to use will be the one that best adapts to the characteristics of the soil: stoniness, depth, organic matter, roots. As a general rule, for a given volume of soil, it is better to extract many small samples than a few large ones, since seed banks often have a very heterogeneous spatial distribution. If it is a matter of studying the seed bank of a given taxon, sampling can focus on those places in the field where seeds can be expected to concentrate, depending on the type of primary and secondary dispersal (e.g. under the cover of parent plants, or within a certain radius from their base, etc.). If the study covers the whole community, plots of a given size (e.g. 1 m2) can be established from which randomly arranged extractions can be made.

There are two compatible ways to find out whether a taxon's seed bank is transient or persistent. The most obvious is to extract the soil shortly before the seed dispersal takes place: its presence in the samples will be a clear indication of the persistent nature of the bank. The other option is to analyse the vertical distribution of seeds in the soil.

Most seeds lack active burial mechanisms, so they do not usually reach strata beyond the first centimeters of the soil, unless they remain in it for a sufficiently long time. On this assumption, a correspondence can be established between the types of seed banks of the classification proposed by Thompson et al. (1997) and their vertical distribution in the soil: (i) transient seed banks accumulate their seeds on the surface (first centimeters); (ii) short-lived persistent seed banks (between 1-5 years) have a representative fraction of seeds in deeper soil strata, but in a significantly smaller quantity than in the superficial stratum; (iii) long-lived persistent seed banks (>5 years) accumulate as many seeds in deep strata as on the surface. A sampling of the soil (natural, without removal) in two strata, from 0 to 4 (or 5) cm and from 4 (or 5) to 8 (or 10) cm, on the same points in each extraction, allows this correspondence to be established. If it is also carried out just before the seeds are dispersed, it makes it possible to define precisely the persistent or transitory nature of the seed bank of a taxon. The morphological characteristics of the seeds, in addition, complement the diagnosis: the seeds of species that form persistent banks usually are of small size and tend to approximate to the spherical form, traits that the natural selection has modulated to facilitate their burial; on the contrary, seeds distant from the sphericity or with appendages that make difficult their burial form transitory banks in the surface of the soil (Thompson et al., 1993).

Analysis of seed content in soil samples. There are two general techniques for counting soil seeds: (i) those based on the emergence of seedlings and (ii) those based on the physical separation of seeds. The first group of techniques requires the cultivation of soil samples. Each emergent seedling corresponds to a viable seed, which will have to be taxonomically identified and then removed, so as not to interfere with the germination and/or emergence of other seeds and/or seedlings. Soil samples should be grown in an enclosure limited to contamination by the arrival of exogenous seeds or disturbance by animals. An umbraculum (enclosure covered with mesh) usually gives better results than a greenhouse, since it effectively reproduces the natural conditions of temperature and light that govern the processes and phenology of germination. Soil samples should first be passed through a coarse-light sieve to remove stones and plant debris, and then spread individually over some type of container (trays), forming a thin layer (no more than 1-2 cm) to facilitate the emergence of small seedlings, on a seedless substrate base (sterile peat, for example) that allows the seedlings to take root until they are identified and removed. The transplant of seedlings to individual flowerpots when they cannot be identified early gives good results, when freeing the samples of developed plants that difficult the manifestation of other seeds. The samples should be cultivated long enough to ensure that the seed bank has been able to express itself fully, which may require up to a complete phenological cycle. Thus, the difficulty in identifying the seedlings, the lethargy of the seeds and the space and time necessary for germination and emergence of the bank in the samples are the main limiting factors of this method.

Ter Heerdt et al. (1996) proposed to do a pre-screening of soil samples by washing on a small light sieve (0.25 mm), so that, without losing seeds, the volume of soil to be handled is reduced and germination is stimulated. With this methodology, the cultivation time required for the expression of the complete seed bank of nemoral ecosystem communities in Central Europe was estimated to be around 6 weeks. However, the effectiveness of this protocol will depend very much on the type of seed dormancy of the dominant species in the community. For example, Ferrandis et al. (1999a, 1999b), in the study of cistáceas-rich Mediterranean shrubs that form huge persistent seed banks with physical lethargy, had to cultivate samples between 1 and 2 years.

The protocols for physical separation of seeds are based on the detection and extraction of seeds from soil samples, usually with the help of sieves and magnifying glass. The factors limiting their effectiveness are the size of the seeds and their viability. Ferrandis et al. (1999a) determined that in the fraction of soil passing through the 0.5 mm mesh sieve, imprecision in seed detection makes this methodology unfeasible. Above this size, it is possible to locate and extract the seeds faithfully, although in general it is very time-consuming. Moreover, not all seeds with a good external appearance are in fact viable: the embryo must be diagnosed by excision of a representative sample of the extracted seeds, either based on their colour and turgidity, or by staining with tetrazolium salts (Ferrandis 1999b). Therefore, physical separation of seeds will be a good option if we work with relatively large seeds. In general, this method is reserved for studies focused on specific taxa, of which we have information on the appearance of the seeds (possibility of identification), size (greater than 0.5 mm) and especially if they have seminal lethargy difficult to overcome. In these cases, the samples are filtered with sieves of light of inferior mesh and superior to their dimensions, to reduce the volume of soil to process. In other cases, especially when the focus of our study is the seed bank of an entire community, we will turn to emergency seedling techniques.

Table 1. - Methodological protocols for the detection of contained seeds
in soil samples (adapted from Ferrandis et al. 2011a)

Separación física

Emergencia de plántulas

Tamizado. Lavado de la muestra por un tamiz con luz de malla superior a las semillas objeto de estudio, para eliminar elementos gruesos y restos orgánicos (raíces, bulbos, hojarasca), y sobre otro con luz de malla inferior, que retenga las semillas.

Reconocimiento de plántulas.
Observación en campo, o siembra de semillas s en macetas, para reconocer las plántulas del taxón en los primeros estadios de desarrollo.

Secado. Secado de la muestra a temperatura ambiente, sobre el propio tamiz de lavado.

Tamizado.Tamizado de la muestra sobre un tamiz con luz de malla superior a las semillas que se estudian, para la eliminación de los elementos gruesos y restos orgánicos (raíces, bulbos, hojarasca), susceptibles de dificultar la emergencia de plántulas; también puede ser efectivo el lavado de las muestras sobre un tamiz fino, que retenga las semillas y permita reducir el volumen de la muestra, con el fin de acortar el periodo de cultivo.

Escrutinio de la muestra. Extendido de la muestra de suelo, pro fracciones o completa, sobre una bandeja, placa Petri, o similar; búsqueda y extracción de semillas con ayuda de pinzas y de lupa si fuera necesario.

 Cultivo en invernadero. Extendido de la muestra en una bandeja, lo más finamente posible (con un grosor nunca superior a 1-2 cm) sobre una capa de sustrato libre de semillas; riego periódico (se recomienda microaspersión) para mantener el suelo constantemente húmedo.

Análisis de viabilidad.
La apariencia externa de las semillas no es un indicador preciso de su viabilidad. Se recomienda inspeccionar el aspecto del embrión (color y estado de hidratación); también se pueden utilizar tinciones de tetrazolio; realizar el ensayo con una muestra representativa de las semillas extraídas del suelo.

Conteo de plántulas. Revisión periódica de las muestras; conteo y retirada de las plántulas tan pronto como se identifiquen; cuando cese la emergencia de plántulas, se recomienda remover el suelo (con cuidado de no mezclarlo con el estrato del sustrato base estéril, para promover la germinación de semillas que pudieran estar enterradas a demasiada profundidad.

 

BIBLIOGRAPHICAL REFERENCES

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BAKKER J.P., A.F. BOS, J. HOOGVELD Y H.J. MULLER. 1991. The role of the seed bank in restoration management of semi-natural grasslands. En Terrestrial and Aquatic Ecosystems: Perturbation and Recovery (O. Ravera Ed.), Ellis Horwood, Nueva York, 449-455.

BASKIN C.C. Y J.M. BASKIN. 1998. Seeds: Ecology, Biogeography, and, Evolution of Dormancy and Germination, Elsevier, Nueva York.

FENNER M. (Ed.). 2000. Seeds: The Ecology of Regeneration in Plant Communities. CABI Publishing, Wallingford.

FERRANDIS P., J.J. MARTÍNEZ-SÁNCHEZ Y J.M. HERRANZ. 1999a. Fire impact on a maquis soil seed bank in Cabañeros National Park (central Spain). Israel Journal of Plant Sciences, 47: 17-26.

FERRANDIS P., J.J. MARTÍNEZ-SÁNCHEZ Y J.M. HERRANZ. 1999b. Effect of fire on hardcoated Cistaceae seed banks and its influence on techniques for quantifying seed banks. Plant Ecology, 144: 103-114.

FERRANDIS P., E. MARTÍNEZ-DURO, M.A. COPETE Y J.M. HERRANZ. 2011a. Propuestas de mejora: metodología para el análisis de los bancos de semillas del suelo de taxones amenazados. En Iriondo J.M. (Ed.). Atlas y Libro Rojo de la Flora Vascular Amenazada de España: Manual de Metodología del Trabajo Corológico y Demográfico, Dirección General de Medio Natural y Política Forestal (Ministerio de Medio Ambiente, y Medio Rural y Marino)-Sociedad Española de Biología de la Conservación de Plantas, Madrid.

FERRANDIS P., M. BONILLA Y L.C. OSORIO. 2011b. Germination and soil seed bank traits of Podocarpus angustifolius Griseb. (Podocarpaceae), a tree species endemic to Cuban mountain rain forests. International Journal of Tropical Biology and Conservation (Revista de Biología Tropical), 59: 1061-1069.

LECK M.A., Y.T. PARKER, Y R.L. Simpson, (Eds) (1989). Ecology of Soil Seed Banks. Academic Press, Orlando, Florida.

TER HEERDT G.N.J., G.L. VERWEIJ, R.M. BEKKER Y J.P. BAKKER. 1996. An improved method for seed-bank analysis: seedling emergence after removing the soil by sieving. Functional Ecology, 10: 144-151.

THOMPSON K. Y J.P. GRIME. 1979. Seasonal variation in the seed banks of herbaceous species in ten contrasting habitats. Journal of Ecology, 67, 893-921.

THOMPSON K., J.P. BAKKER Y R.M. BEKKER. 1997. The soil seed banks of North West Europe: methodology, density and longevity. Cambridge University Press, Cambridge.

THOMPSON K., S.R. BAND Y J.G. HODGSON. 1993. Seed size and shape predict persistence in soil, Functional Ecology, 2: 236-241.

 


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Copyright (c) 2019 Pablo Ferrandis