Revista Cubana de Ciencias Forestales. 2020; mayo-agosto 8(2): 333-343

Translated from the original in spanish

Reproductive capacity of Pinus caribaea Morelet var. caribaea Barret y Golfari in Marbajita´s the seed orchard

Capacidad reproductiva de Pinus caribaea Morelet var. caribaea Barret y Golfari en la unidad de producción de semilla mejorada Marbajita

Capacidade reprodutiva de Pinus caribaea Morelet var. caribaea Barret e Golfari na unidade de produção de sementes melhorada de Marbajita

Claudia María Pérez Reyes^1* https://orcid.org/0000-0002-3690-3119

Mumboko Wilfrid Fuentes González² https://orcid.org/0000-0002-2080-6285

Gretel Geada López¹ https://orcid.org/0000-0002-8421-0624

¹Universidad de Pinar del Río "Hermanos Saíz Montes de Oca", Pinar del Río, Cuba.
²Empresa Agroforestal La Palma, UEB Marbajita, Pinar del Río, Cuba.

^*Correspondence author: cludia.maria@upr.edu.cu

Received: June 5^th, 2020
Approved: June 16^th, 2020.

ABSTRACT

The study allows to evaluate the reproductive capacity of Pinus caribaea through the behavior of the reproductive indicators during two consecutive years. A sample of 109 cones was taken from trees in the improved seed production unit Marbajita-Cajálbana, corresponding to an area of natural pine forests in the municipality of La Palma. The characterization of their cones was made from their dimensions, besides determining the variables related to seed production such as fertile and infertile flakes, vain and full seeds, seed potential and efficiency, proportion of full and vain seeds, inbreeding index and germination percentage. Results related to cone length showed significant differences for the years evaluated, with the lowest values reported in 2016. High rates of inbreeding were evidenced above 12 %, acceptable for the species and for areas intended to produce improved seed. Germination was higher in 2017 with 100 %.

Keywords: Reproductive indicators; Pinus caribaea var. caribaea; Reproductive capacity.

RESUMEN

El estudio permite evaluar la capacidad reproductiva de Pinus caribaea a través del comportamiento de los indicadores reproductivos durante dos años consecutivos. Se tomó una muestra de 109 conos provenientes de árboles de la unidad de producción de semilla mejorada Marbajita-Cajálbana, correspondiente a un área de pinares naturales del municipio de La Palma. Se realizó la caracterización de sus conos a partir de sus dimensiones, además de determinar las variables relacionadas a la producción de semillas como escamas fértiles e infértiles, semillas vanas y llenas, potencial y eficiencia de semillas, proporción de semillas llenas y vanas, índice de endogamia y porcentaje de germinación. Los resultados relacionados a la longitud del cono mostraron diferencias significativas para los años evaluados, reportándose los valores más bajos en 2016. Se evidenciaron altos índices de endogamia por encima de un 12 %, admisible para la especie y para áreas que tiene la finalidad de producir semilla mejorada. La germinación fue superior en 2017 con un 100 %.

Palabras clave: Indicadores reproductivos; Pinus caribaea var. caribaea; Capacidad reproductiva.

RESUMO

O estudo permite avaliar a capacidade reprodutiva do Pinus caribaea através do comportamento dos indicadores reprodutivos durante dois anos consecutivos. Uma amostra de 109 cones foi retirada das árvores da unidade de produção de semente melhorada Marbajita-Cajálbana, correspondente a uma área de pinhais naturais no município de La Palma. A caracterização dos seus cones foi feita a partir das suas dimensões, além de determinar as variáveis relacionadas com a produção de sementes, tais como escalas férteis e inférteis, sementes vazias e cheias, potencial e eficiência das sementes, proporção de sementes cheias e vazias, índice de endogamia e percentagem de germinação. Os resultados relacionados com o comprimento do cone mostraram diferenças significativas para os anos avaliados, tendo os valores mais baixos sido reportados em 2016. Foram evidenciadas taxas elevadas de endogamia acima dos 12 %, aceitáveis para as espécies e para as áreas destinadas a produzir sementes melhoradas. A germinação foi mais elevada em 2017 com 100 %.

Palavras-chave: Indicadores reprodutivos; Pinus caribaea var. caribaea; Capacidade reprodutiva.

INTRODUCTION

Pinus caribaea var. caribaea is one of the species recognized as a priority in the management and plantation within the development plans until 2030, due to its great plasticity of adaptation to different environments (García-Quintana et al., 2013), as well as the quality of its wood for numerous uses. All this requires the increase of its plantations to obtain genetic quality seed, so the source of the material to be propagated is of special interest.

In this sense, in the 1960s work started on the selection and creation of seed sources for this species (García-Quintana et al., 2007), based on genetic improvement programs by mass and individual selection, thus beginning research for the selection and improvement of the main seed sources.

Seed orchards or stands are areas created or selected for the purpose of producing seeds with a certain degree of progress in the given characteristic, which is the object of forest improvement (White et al., 2007). Those are determined or selected according to morphological parameters, with economic interest; but the capacity of these trees and areas to produce quality seeds is rarely evaluated (Finkeldey et al., 2007), so from a productive point of view it is not only important that they are superior specimens, but also producers of seed similar to the potential (Eriksson et al., 2013).

P. caribaea var. caribaea has established seed production units, both by the method of mass selection (Meseta de Cajálbana) and individual (mainly: Malas Aguas, Estación experimental de Viñales), with the aim of increasing timber volumes.

Marbajita is one of the seed stands, in the Meseta de Cajálbana, was created in the 1960s from mass selection in a natural pine forest. During the 90's, more than 60% of its area was burned, so it was restored from superior trees of the same origin; it represents one of the main sources of seed for the species in Pinar del Río and for the country.

This stand reports the best behaviors in the nursery phase (technical reports, MINAG). On the other hand, the production of cones and seeds, as well as the quality certificates of the seed lots show a reduction, both in the quantity of seed and in the germination capacity.

Measures of reproductive characteristics, along with genetic diversity, can serve as indicators of reproductive and genetic status to determine and monitor the viability of populations at risk due to small population size (Delgado-Valerio et al., 2013) and low density of trees within the forest (Quiroz-Vázquez et al., 2017; Ramírez-Mandujano et al., 2017).

Reproductive Indicators (RIs) are points of reference that provide qualitative or quantitative information on seed sources. They are also basic tools for monitoring populations (Flores et al., 2012), especially those designed to meet the needs of forestry (Pérez-Reyes and Geada-López, 2020; Santos-Sánchez et al., 2018).

The objective of this study was to evaluate the reproductive capacity through the behavior of the reproductive indicators in a seed mass (Marbajita-Cajálbana) of P. caribaea var. caribaea, in two years.

MATERIALS AND METHODS

Samples of 60 and 49 cones of the Marbajita-Cajálbana seed mass were collected in July 2016 and 2017. The seed production unit belongs to the UEB of the municipality of La Palma (Figure 1), presents the category of improved seed production and corresponds to an area of natural pine forest treated for this purpose. The seed stand occupies a total of 72 ha of pine forests, with an average height of 20-25 meters.

Figure 1. - Location of the Marbajita-Cajálbana seed stand

The cones were dried at room temperature for one month until the beginning of their opening and seed extraction. Their length was then determined and they were then dissected to count the Total Number of Flakes (NoE), in addition to Fertile Flakes (EF) and Infertile Flakes (Einf), Full Seeds (SLL) and Vane Seeds (SV). The seed certification test was carried out under Cuban Standard NC: 71-04/87.

The indicators were calculated using Bramlett's formulas (Quiroz-Vázquez et al., 2017). To infer the existence of inbreeding in the seed mass, a germination test was carried out together with the evaluation of the viability of the seeds. The germination test was established in silica sand (glass containers), for 45 days. Germinated seed was considered to be seed whose radicle length exceeded the length of the embryo. Seed viability was determined from colorimetric tests.

Biometric Analysis: an analysis of non-parametric Kruskal-Wallis variance and a range comparison test were used to detect differences between collection years.

RESULTS AND DISCUSSION

Cone dimensions showed differences in both collection years (Table 1). This value is generally associated with seed availability and germination capacity, which indicates that trees with larger cone sizes produce larger seeds (Flores-López et al., 2005). Seed weight is also important, there is evidence in conifers where heavier seeds are associated with larger seeds, and therefore produce more robust seedlings at least during the first year of life (Flores-López et al., 2005). However, it has been found that cone and seed variables can vary between populations, between individuals and between regions, for species with a wider distribution (Boratynska et al., 2005).

Although the collected strobile in 2016 were larger than those collected in 2017, the latter yielded the best indicators related to seed production, the average Seed Potential (SP), Fertile Flakes (EF) and developed seeds (Table 1).

Flores-López et al., (2012) and Quiroz-Vázquez et al., (2017) propose that seed potential is an indicator related to the genetic structure of populations or areas of seed production, and this in turn represents the effectiveness of the process of pollination, fertilization and maturation of the seed (Fernando, 2014). In addition, it is the most evident expression of the effective population size that determines the proportion of pollen needed to make allogamy effective (Fernando, 2014; Flores-López, 2014).

Forest trees, under natural conditions, do not produce seeds regularly, but rather in variable periods of years, which can be from one to seven years or more; this characteristic is associated with some factors of the climate and maturity of the tree (Alba-Landa et al., 2005). Differences between seed years have been reported in several conifers, such as P. greggii (Alba-Landa et al., 2005), whose variations have been attributed primarily to abiotic factors such as humidity (rain), wind and drought events.

The indicators associated with full and vain seeds are significantly different between crops, but not in their proportions. One possible cause is associated with the absence of cross-pollination and fertilization, which affects the efficiency of the seed and is to be expected in small populations or in solitary trees. The results show that there is a high percentage of vain seeds; this value is usually recommended to be below 20 % (Sorensen, 2001; Ledig et al., 2002) and for conifers in seed source areas it is accepted that it is around 12 % as an indicator of unaffected depression by inbreeding (Sorensen, 2001; Ledig et al., 2002). The results in two years show that this value was higher than expected for seed-producing areas and higher than that obtained for the species in a non-certified seed stand (Pérez-Reyes, unpublished data).

In this sense, in the seed stand (Marbajita) to be evaluated, the differences obtained in the indicators and germination must be related to environmental factors such as drought and high temperatures in the months of April-June 2013 to 2016, which correspond to the years of development of female strobile its pollination and seed formation. However, there were no differences between the proportion of SLLP.

Table 1. - Behavior of reproductive indicators and cone during two harvests

Note: averages with different letters have significant differences at P < 0,05

Owens et al., (2005, 2008); Flores-López et al., (2005) report for Pinus and Picea a decrease in seed potential, which could be associated with inbreeding, especially for stands with small effective sizes, given the low effectiveness of morphological mechanisms to prevent self-crossing and given that genetic incompatibility mechanisms have not been developed in this taxonomic group (Bower and Aitken, 2007). It is recognized that large population sizes are a requirement for seed stands and should correspond to the 100/1000 rule (Frankham et al., 2014), so as to ensure cross-pollination and the conservation of genetic variability, especially in the case of natural stands converted into seed orchards.

Seed efficiency is the most representative variable within seed production (Bramlet et al., 1977; Mosseler, 2001). This indicator varied substantially between years, with the lowest value reported in 2016, indicating that there may have been reduced pollen availability. The low seed efficiency is generally attributed to four causes: low pollination, presence of lethal genes, insect damage (Bustamante-García et al., 2012) and low pollen availability with asynchrony in pollen release (Fernando, 2014); however, studies in tropical pines are scarce (Fernando, 2014) and do not allow to reach a regularity in the causes. The results obtained are similar to those recorded for P. patula (Alba-Landa et al., 1999), although lower than species such as P. greggii (Alba-Landa et al., 2005) and P. hartwegii (Alba-Landa et al., 2003) in natural stands and higher than P. oocarpa (Isaza et al., 2002) and P. sylvestris (Sivacioðlu and Ayan, 2008) for a seed orchard.

According to the development of the cone from flowering to formation and maturity of the cone, there are also losses of strobile, which were not considered in the study. Strobile losses can be up to 50 % or more of the seed harvest and the total seed production can be evaluated by combining the cone harvest life tables (Owens y Fernando, 2007; Fernando, 2014).

The inbreeding estimator (IEND) is extremely important from a silvicultural point of view; it reflects stand health, pollen availability and the amount of viable seeds to be produced (White et al., 2007). The results show relatively high values (≥12 %), for areas that are intended to produce improved seed. Inbreeding depression generates low percentages in germination, which in turn limits the production of vigorous seedlings and produces an increase in abnormal seedlings. The effects associated with inbreeding depression reduce the number of filled seeds and increase the number of vain seeds, production of weak, albino seedlings, with less ability to survive (Ramírez-Mandujano et al., 2017). These results show that the behavior of this indicator was similar in the two years; however, germination was higher in 2017 with 100 % of seeds germinated, and in 2016 it only reached 50 %. This makes us think that it was not inbreeding that caused the low germination, but the possible low availability of pollen or favourable conditions during the long process of formation and maturation of the seed in the species three years (Fernando, 2014).

The year 2016 reports the worst reproductive indicators, corresponding to the lowest germination values, but no abnormal seedlings were detected in any year evaluated. This, together with the behavior of the reproductive indicators, indicates that the climate conditions during the years of formation, pollination and maturation were the causes of the marked differences between the years (Fernando, 2014). Therefore, monitoring studies of seed sources through indicators are necessary to correlate their changes with bioclimatic variables and to document the incidence of climate change on seed production.

A comparison of the two years showed a better performance of the indicators in 2017; however, this did not correspond to the results obtained for the length of the cone.

The differences obtained in the reproductive indicators could be related to climatic factors, such as the prolonged drought 2013-2015.

In 2016 the worst reproductive indicators are reported, which in turn corresponds to the lowest by hundreds of germination for the species.

REFERENCES

ALBA-LANDA J., M.H. LILIA C. Y C. G. ADRIANA A. 1999. Potencial de producción de semilla de la progenie de un huerto semillero de segunda generación de selección de Pinus patula Schl. et Cham. en el estado de Veracruz. ISIMA. Durango, México. Pp. 133-134.

ALBA-LANDA J., A. APARICIO-RENTERÍA Y J. MÁRQUEZ-RAMÍREZ. 2003. Potencial y Eficiencia de producción de semillas de Pinus hartwegii Lindl. de dos poblaciones de México. Foresta Veracruzana, vol.5, no 1, p. 23-26. Disponible en: https://www.researchgate.net/publication/228607143_POTENCIAL_Y_EFICIENCIA_DE_PRODUCCION_DE_SEMILLAS_DE_Pinus_hartwegii_Lindl_DE_DOS_POBLACIONES_DE_MEXICO

ALBA-LANDA, J., RAMÍREZ, J.M. y CORTINA, H.S.B., 2005. Potencial de producción de semillas de Pinus greggii engelm. en tres cosechas de una población ubicada en Carrizal Chico, Zacualpan Veracruz, México. Foresta Veracruzana [en línea], vol. 7, no. 2, pp. 37-40. [Consulta: 9 junio 2020]. ISSN 1405-7247. Disponible en: https://www.redalyc.org/articulo.oa?id=49770206.

BORATYÑSKA, K., MARCYSIAK, K. y BORATYÑSKI, A., 2005. Pinus mugo (Pinaceae) in the Abruzzi Mountains: high morphological variation in isolated populations. Botanical Journal of the Linnean Society [en línea], vol. 147, no. 3, pp. 309-316. [Consulta: 9 junio 2020]. ISSN 0024-4074. DOI 10.1111/j.1095-8339.2005.00374.x. Disponible en: https://academic.oup.com/botlinnean/article/147/3/309/2420526.

BOWER, A.D. y AITKEN, S.N., 2007. Mating system and inbreeding depression in whitebark pine (Pinus albicaulis Engelm.). Tree Genetics & Genomes [en línea], vol. 3, no. 4, pp. 379-388. [Consulta: 9 junio 2020]. ISSN 1614-2950. DOI 10.1007/s11295-007-0082-4. Disponible en: https://doi.org/10.1007/s11295-007-0082-4.

BRAMLETT, D.L., BELCHER, E.W., DEBARR, G.L., HERTEL, G.D., KARRFALT, R.P., LANTZ, C.W., MILLER, T., WARE, K.D. y YATES, H.O., 1977. Cone Analysis of Southern Pines - A Guidebook. Gen. Tech. Rep. SE-13. Asheville, NC: U.S. Department of Agriculture, Forest Service, Southeastern Forest Experiment Station. 32 p. [en línea], vol. 013. [Consulta: 9 junio 2020]. Disponible en: https://www.srs.fs.usda.gov/pubs/808.

BUSTAMANTE-GARCÍA, V., PRIETO-RUÍZ, J.Á., MERLÍN-BERMUDES, E., ÁLVAREZ-ZAGOYA, R., CARRILLO-PARRA, A. y HERNÁNDEZ-DÍAZ, J.C., 2012a. Potencial y eficiencia de producción de semilla de Pinus engelmannii Carr., en tres rodales semilleros del estado de Durango, México. Madera y bosques [en línea], vol. 18, no. 3, pp. 7-21. [Consulta: 9 junio 2020]. ISSN 1405-0471. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S1405-04712012000300002&lng=es&nrm=iso&tlng=es.

BUSTAMANTE-GARCÍA, V., PRIETO-RUÍZ, J.Á., MERLÍN-BERMUDES, E., ÁLVAREZ-ZAGOYA, R., CARRILLO-PARRA, A. y HERNÁNDEZ-DÍAZ, J.C., 2012b. Potencial y eficiencia de producción de semilla de Pinus engelmannii Carr., en tres rodales semilleros del estado de Durango, México. Madera y bosques [en línea], vol. 18, no. 3, pp. 7-21. [Consulta: 9 junio 2020]. ISSN 1405-0471. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S1405-04712012000300002&lng=es&nrm=iso&tlng=es.

DELGADO VALERIO, P., NÚÑEZ MEDRANO, J., ROCHA GRANADOS, M.C. y MUÑOZ FLORES, H.J., 2013. Variación genética de dos áreas semilleras de pino establecidas en el estado de Michoacán. Revista mexicana de ciencias forestales [en línea], vol. 4, no. 18, pp. 104-115. [Consulta: 9 junio 2020]. ISSN 2007-1132. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S2007-11322013000400008&lng=es&nrm=iso&tlng=es.

DÍAZ, A.R.Á., 2017. Diagnóstico de dos fuentes semilleras de Pinus tropicalis Morelet y Eucalyptus urophylla S.T. Blake en la Empresa Forestal Integral "Macurije". Ciencias Forestales y Ambientales [en línea], vol. 2, no. 2, pp. 135-149. [Consulta: 9 junio 2020]. ISSN 2310-3426. Disponible en: http://cifam.upr.edu.cu/index.php/cifam/article/view/95.

ERIKSSON, G., EKBERG, I. y CLAPHAM, D., 2013. Genetics Applied to Forestry An Introduction [en línea]. S.l.: Elanders Sverige AB. Disponible en: https://pub.epsilon.slu.se/12141/1/eriksson_et_al_150423.pdf.

FINKELDEY, R. y HATTEMER, H.H., 2006. Tropical Forest Genetics [en línea]. S.l.: Springer Science & Business Media. ISBN 978-3-540-37398-8. Disponible en: https://books.google.com.cu/books/about/Tropical_Forest_Genetics.html?id=1dVnC7ubkxYC&redir_esc=y.

FLORES LÓPEZ, C., 2014. Líneas para la conservación de los recursos genéticos de Picea mexicana Martínez y Picea martinezii Patterson [en línea]. Tesis. Pinar del Río, Cuba: Universidad de Pinar del Río Hermanos Saíz Montes de Oca. Facultad de Forestal y Agronomía. Centro de Estudios Forestales. [Consulta: 9 junio 2020]. Disponible en: https://rc.upr.edu.cu/jspui/handle/DICT/2170.

FLORES LÓPEZ, C., GEADA LÓPEZ, G., UPTON, J. y LÓPEZ-RAMÍREZ, E., 2012. Producción de semillas e indicadores reproductivos en poblaciones naturales de Picea martinezii T.F. Patterson. Forestal Baracoa, vol. 31, pp. 48-52. Disponible en: https://www.researchgate.net/publication/286777475_Produccion_de_semillas_e_indicadores_reproductivos_en_poblaciones_naturales_de_Picea_martinezii_TF_Patterson

FLORES-LOPÉZ, C., LÓPEZ-UPTON, J. y VARGAS-HERNÁNDEZ, J.J., 2005. Indicadores reproductivos en poblaciones naturales de Picea mexicana Martínez. Agrociencia [en línea], vol. 39, no. 1, pp. 117-126. ISSN 1405-3195. Disponible en: https://www.redalyc.org/pdf/302/Resumenes/Resumen_30239111_1.pdf.

FRANKHAM, R., BRADSHAW, C.J.A. y BROOK, B.W., 2014. Genetics in conservation management: Revised recommendations for the 50/500 rules, Red List criteria and population viability analyses. Biological Conservation [en línea], vol. 170, pp. 56-63. [Consulta: 9 junio 2020]. ISSN 0006-3207. DOI 10.1016/j.biocon.2013.12.036. Disponible en: http://www.sciencedirect.com/science/article/pii/S0006320713004576.

GARCÍA-QUINTANA, Y., ÁLVAREZ-BRITO, A. y GUÍZAR-NOLAZCO, E., 2007. ENSAYO DE PROCEDENCIAS DE Pinus caribaea var. caribaea EN ALTURAS DE PIZARRAS, VIÑALES, PINAR DEL RÍO, CUBA. Revista Chapingo serie ciencias forestales y del ambiente [en línea], vol. 13, no. 2, pp. 125-129. [Consulta: 9 junio 2020]. ISSN 2007-4018. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S2007-40182007000200125&lng=pt&nrm=iso&tlng=es.

ISAZA, N., DVORAK, W. y UPTON, J., 2002. Producción de semillas del género Pinus en huertos y rodales semilleros de Smurfit Cartón de Colombia. [en línea]. Cali, Colombia: Smurfit Cartón de Colombia. Disponible en: https://www.researchgate.net/publication/289251403_Produccion_de_semillas_del_genero_Pinus_en_huertos_y_rodales_semilleros_de_Smurfit_Carton_de_Colombia.

LEDIG, F.T., HODGSKISS, P.D. y JACOB-CERVANTES, V., 2002. Genetic diversity, mating system, and conservation of a Mexican subalpine relict, Picea mexicana Martínez. Conservation Genetics [en línea], vol. 3, no. 2, pp. 113-122. [Consulta: 9 junio 2020]. ISSN 1572-9737. DOI 10.1023/A:1015297621884. Disponible en: https://doi.org/10.1023/A:1015297621884.

OWENS, J., BENNETT, J. y L'HIRONDELLE, S.J., 2005. Pollination and cone morphology affect cone and seed production in lodgepole pine seed orchards. Canadian Journal of Forest Research [en línea], vol. 35, pp. 383-400. DOI 10.1139/x04-176. Disponible en: https://www.researchgate.net/publication/237866256_Pollination_and_cone_morphology_affect_cone_and_seed_production_in_lodgepole_pine_seed_orchards.

OWENS, J. y FERNANDO, D., 2007. Pollination and seed production in western white pine. Canadian Journal of Forest Research [en línea], vol. 37, pp. 260-275. DOI 10.1139/X06-220. Disponible en: https://www.researchgate.net/publication/233555628_Pollination_and_seed_production_in_western_white_pine.

OWENS, J.N., KITTIRAT, T. y MAHALOVICH, M.F., 2008. Whitebark pine (Pinus albicaulis Engelm.) seed production in natural stands. Forest Ecology and Management [en línea], vol. 255, no. 3, pp. 803-809. [Consulta: 9 junio 2020]. ISSN 0378-1127. DOI 10.1016/j.foreco.2007.09.067. Disponible en: http://www.sciencedirect.com/science/article/pii/S0378112707007396.

PÉREZ REYES, C.M., GEADA LÓPEZ, G., PÉREZ REYES, C.M. y GEADA LÓPEZ, G., 2020. Producción de semillas e indicadores reproductivos de Pinus tropicalis Morelet en un huerto semillero. Revista Cubana de Ciencias Forestales [en línea], vol. 8, no. 1, pp. 129-137. [Consulta: 9 junio 2020]. ISSN 2310-3469. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S2310 -34692020000100129&lng=es&nrm=iso&tlng=es.

QUINTANA, Y.G., VICHOT, M.B., TORRES, G.P. y ROJAS, A.E.A., 2013. Ecología, silvicultura y conservación de los pinares de la región occidental de Cuba (Pinus caribaea Morelet var. caribaea y Pinus tropicalis Morelet) [en línea]. S.l.: Servicio de Publicaciones. [Consulta: 9 junio 2020]. ISBN 978-84-9717-247-9. Disponible en: https://dialnet.unirioja.es/servlet/libro?codigo=652135.

QUIROZ-VÁZQUEZ, R.I., LÓPEZ-UPTON, J., CETINA-ALCALÁ, V.M., ÁNGELES-PÉREZ, G., QUIROZ-VÁZQUEZ, R.I., LÓPEZ-UPTON, J., CETINA-ALCALÁ, V.M. y ÁNGELES-PÉREZ, G., 2017. Capacidad reproductiva de Pinus pinceana Gordon en el límite sur de su distribución natural. Agrociencia [en línea], vol. 51, no. 1, pp. 91-104. [Consulta: 9 junio 2020]. ISSN 1405-3195. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S1405-31952017000100091&lng=es&nrm=iso&tlng=es.

RAJORA, O.P. y MOSSELER, A., 2001. Challenges and opportunities for conservation of forest genetic resources. Euphytica [en línea], vol. 118, no. 2, pp. 197-212. [Consulta: 9 junio 2020]. ISSN 1573-5060. DOI 10.1023/A:1004150525384. Disponible en: https://doi.org/10.1023/A:1004150525384.

SANTOS SÁNCHEZ, O.O., GONZÁLEZ TAGLE, M.A., LÓPEZ AGUILLÓN, R., SANTOS SÁNCHEZ, O.O., GONZÁLEZ TAGLE, M.A. y LÓPEZ AGUILLÓN, R., 2018. Producción de semilla y potencial biológico de tres especies de Pinus en Durango. Revista mexicana de ciencias forestales [en línea], vol. 9, no. 50, pp. 538-549. [Consulta: 9 junio 2020]. ISSN 2007-1132. DOI 10.29298/rmcf.v9i50.264. Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_abstract&pid=S2007-11322018000600538&lng=es&nrm=iso&tlng=es.

SIVACIOGLU, A. y AYAN, S., 2009. Evaluation of seed production of scots pine (Pinus sylvestris L.) clonal seed orchard with cone analysis method. African Journal of Biotechnology [en línea], vol. 7, pp. 4393-4399. Disponible en: https://www.researchgate.net/publication/228773598_Evaluation_of_seed_production_of_scots_pine_Pinus_sylvestris_L_clonal_seed_orchard_with_cone_analysis_method.

SORENSEN, F.C., 2001. Effect of Population Outcrossing Rate on Inbreeding Depression in Pinus contorta var. murrayana Seedlings. Scandinavian Journal of Forest Research [en línea], vol. 16, no. 5, pp. 391-403. [Consulta: 9 junio 2020]. ISSN 0282-7581. DOI 10.1080/02827580152632784. Disponible en: https://doi.org/10.1080/02827580152632784.

WHITE, T.L., ADAMS, W.T. y NEALE, D.B., [sin fecha]. Forest Genetics [en línea]. S.l.: CABI. ISBN 978-1-84593-286-2. Disponible en: https://books.google.com.cu/books/about/Forest_Genetics.html?id=UHZCeg4BqtkC&redir_esc=y.

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